A catalase is a common enzyme found in nearly all living organisms exposed to oxygen. It catalyzes the decomposition of water and oxygen. It is important in protecting the cell from oxidative damage. 

 Procedure;

1.transfer a small amount of bacteria colony into a surface of clean, dry glass slide using a loop. 

2.place a drop of 3% hydrogen peroxide on the slide and mix. 

3.A positive result is the rapid evolution of oxygen(within 5-10sec) as evidenced by bubbling. 

4.A negative result no bubbles.