Coagulase Test
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Coagulase Test

Coagulase Test

5 years ago

~0.6 mins read
This is a laboratory procedure used to differentiate staphylococcus aureus (positive)  which produces the enzyme coagulase, from Staphylococcus epidermis and S. Saprophyticus(negative)  which do not produce coagulase. 
  Principle: coagulase is an enzyme like protein and causes plasma to clot by converting fibrinogen to fibrin. 
 Procedure(slide test) ;
1.place a drop of physiological saline on each end of a slide, or on 2 separate slides. 
2.with the loop, emulsify a portion of the isolated colony in each drops to make 2 thick suspensions. 
3.Add a drop of human or rabbit plasma to one of the suspensions, and mix gently. 
4.looking for clumping of the organisms within 10seconds.
5.No plasma is added to the second suspension to differentiate any granular appearance of the organism from true coagulase clumping. 

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